Thp1 sting
WebResistance to various therapies, including novel immunotherapies, poses a major challenge in the management of breast cancer and is the leading cause of treatment failure. Bidirectional communication between breast cancer cells and the tumour microenvironment is now known to be an important contributor to therapy resistance. Several studies have … WebNational Center for Biotechnology Information
Thp1 sting
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WebApr 12, 2024 · THP1-ACE2, THP1-cGAS KO-ACE2, and THP1-STING KO-ACE2 cells were generated by transduction of THP1, THP1-cGAS KO, and THP1-STING KO cells with … WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase …
WebApr 2, 2024 · Interestingly, when we compared the precipitation of STING and STIM1 with ISD017 in Wt, STING KO, and STIM1 KO THP1 macrophages, we observed that STIM1 … WebIRF3 can then bind to interferon-stimulated responsive elements (ISRE) in the nucleus and leads to IFN-α/β production. The IRF reporter (Luc)-THP-1 cell line is highly responsive to STING and CDS ligands. Purchase of this …
WebWherein the increased immune response comprises an increase in the level or activity of NFκB, IRF or STING in an immune cell. In one embodiment, the immune cell is a THP-1 cell. In one ... 8B shows NFkB activity in THP1 monocytes co-cultured with HT1080 fibrosarcoma cells treated with Erastin alone or in combination with a ferroptosis ... WebMay 20, 2024 · IRF luciferase reporter assays in (a) THP1-STING-R232, THP1-STING-H232, THP1-STING-HAQ, and THP1-STING-KO cells. Cells were stimulated for 24 h with the …
WebProject: Identification and functional assessment of human homologues of cGAS, in particular other members of the MAB21 family. cGAS is an intracellular sensor that detects pathogenic dsDNA. If viral or bacterial dsDNA is sensed by cGAS, it triggers a cellular immune (Interferon-Beta/IFNβ) response via the adaptor protein STING. Lab work (2…
WebJun 1, 2024 · Addition of suramin to THP1 cells reduced the levels of IFN-β mRNA and protein. Suramin did not inhibit lipopolysaccharide- or Pam3CSK4-induced IL-6 mRNA … st henry catholic church cleveland ohioWebIn order to study which pathway that the RNA-DNA complex activates the immune response, the researchers generatedMAVS, cGAS,STING knockout THP-1 cell lines, and introduced … st henry catholic church buckeye azWebFigure 1 IFN-γ and LPS cooperatively induce CCL2 in monocytic cells/macrophages. THP-1 monocytic cells, primary monocytes, and THP-1-derived macrophages were treated for 24h with IFN-γ (10 ng/mL) alone or in combination with LPS (10 ng/mL). Total RNA was extracted and Ccl2 mRNA expression was quantified by real-time RT-PCR. st henry catholic church chicago ilWebApr 18, 2024 · InvivoGen 에서는 innate immunity-specific signaling pathway 연구를 위한 가장 다양한 cell line을 공급하고 있습니다. SEAP(secreted embryonic alkaline phosphatase) 혹은 Luciferase가 단독 혹은 동시에 발현되며, 세포 밖으로 분비되는 결과를 측정하게 됩니다. 간단하고 쉽게 detection을 하기 위한 reagent를 개발하여 함께 제공하고 ... st henry catholic church daytonWeb3.2. cGAMP and nystatin induced type-I IFN production in THP1 cells 42 3.2.1. Detection of IFN-I in supernatant from THP-1 macrophages cells 42 4. Discussion 45 4.1. Conclusion … st henry catholic church erlanger kyWebJul 1, 2024 · To further assess its efficacy on STING-dependent interferon production, 2'3'-cGAMP was added to the culture medium of a high ENPP1-expressing cell line MDA-MB … st henry catholic church facebookWeb已发现cgas-cgamp-sting途径是对哺乳动物细胞的细胞质中dna的存在的细胞先天免疫应答的一部分。 已鉴定了许多细胞质DNA或RNA的先天传感器(innate sensors)。 参见Barber GN，STING-dependent cytosolic DNA sensing pathways，Trends inimmunology 35：88 … st henry catholic church in buckeye az